OK, I generally don't care for GUIDE GUI's, but here's a really simple example:
function varargout = untitled(varargin)
% UNTITLED M-file for untitled.fig
% UNTITLED, by itself, creates a new UNTITLED or raises the existing
% singleton*.
%
% H = UNTITLED returns the handle to a new UNTITLED or the handle to
% the existing singleton*.
%
% UNTITLED('CALLBACK',hObject,eventData,handles,...) calls the local
% function named CALLBACK in UNTITLED.M with the given input arguments.
%
% UNTITLED('Property','Value',...) creates a new UNTITLED or raises the
% existing singleton*. Starting from the left, property value pairs are
% applied to the GUI before untitled_OpeningFcn gets called. An
% unrecognized property name or invalid value makes property application
% stop. All inputs are passed to untitled_OpeningFcn via varargin.
%
% *See GUI Options on GUIDE's Tools menu. Choose "GUI allows only one
% instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES
% Edit the above text to modify the response to help untitled
% Last Modified by GUIDE v2.5 18-Jun-2013 08:52:25
% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name', mfilename, ...
'gui_Singleton', gui_Singleton, ...
'gui_OpeningFcn', @untitled_OpeningFcn, ...
'gui_OutputFcn', @untitled_OutputFcn, ...
'gui_LayoutFcn', [] , ...
'gui_Callback', []);
if nargin && ischar(varargin{1})
gui_State.gui_Callback = str2func(varargin{1});
end
if nargout
[varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
gui_mainfcn(gui_State, varargin{:});
end
% End initialization code - DO NOT EDIT
end
% --- Executes just before untitled is made visible.
function untitled_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject handle to figure
% eventdata reserved - to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% varargin command line arguments to untitled (see VARARGIN)
% Choose default command line output for untitled
handles.output = hObject;
% Update handles structure
guidata(hObject, handles);
% UIWAIT makes untitled wait for user response (see UIRESUME)
% uiwait(handles.figure1);
end
% --- Outputs from this function are returned to the command line.
function varargout = untitled_OutputFcn(hObject, eventdata, handles)
% varargout cell array for returning output args (see VARARGOUT);
% hObject handle to figure
% eventdata reserved - to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Get default command line output from handles structure
varargout{1} = handles.output;
end
% --- Executes on button press in pushbutton1.
function pushbutton1_Callback(hObject, eventdata, handles)
% hObject handle to pushbutton1 (see GCBO)
% eventdata reserved - to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% get a handle to the text field
textDisp = get(handles.text1,'String');
if( strcmp(textDisp{1},'Static Text' ) )
set(handles.text1,'String',{'Checkout my new text'});
elseif( strcmp(textDisp{1},'Checkout my new text') )
set(handles.text1,'String',{'Clicked it 1 time'})
else
dd = sscanf(textDisp{1},'Clicked it %d time');
dd = dd + 1;
set(handles.text1,'String',{['Clicked it ' num2str(dd) ' time']})
end
end
It's going to run off a really simple figure that looks like this:

Every time the button is pushed, it calls the pushbutton1_Callback with the hObject, handle of the object that caused the callback, the button, eventdata, in this case it'll be empty, and the handles structure which has a handle to everything in the GUI in this case. Merely do your image processing in the callback function or whatever and it should allow you to get where you're going. HTH!