Abstract: The embodiments of the disclosure provide a method for determining a two-eye gaze point and a host. The method includes: obtaining a first gaze point of a first eye on a reference plane and obtaining a second gaze point of a second eye on the reference plane; and determining a two-eye gaze point via combining the first gaze point and the second gaze point.

Abstract: Provided herein is a method of detecting microorganisms in liquid samples using image features, such as time profiles of object light scattering intensity and time profiles of object position. Related methods, devices, systems, and other aspects are also provided.

Abstract: A probe tip landing method for a measurement system is provided. The probe tip landing method includes performing a first descending operation to lower a probe toward a sample by a first descending distance; performing a second descending operation to lower the probe toward the sample; and performing an inspection operation during the second descending operation. The inspection operation includes an imaging operation, scanning the sample to obtain a first image including a probe tip of the probe; and a determining operation, checking the first image to determine that in the first image, whether a region connected with the probe tip becomes bright. The probe tip landing method further includes in response to the region connected with the probe tip in the first image becoming bright, determining that the probe has contacted a surface of the sample and the probe has landed successfully.

Abstract: A method for nonrigid mosaicking of images, including: obtaining, using a processor, a plurality of images including a first image and a second image of a sample, the first image and the second image containing information from partially overlapping portions of the sample; identifying, using the processor, at least one feature match between the first image and the second image; estimating, using the processor, a deformation between the first image and the second image based on identifying the at least one feature match; and combining, using the processor, the first image and the second image based on estimating the deformation between the first image and the second image.

Abstract: An apparatus for feedback utilization in automated scanning systems, the apparatus including a scanning system and a computing device configured to extract a unique identifier from the slide label located on the at least a slide using the scanning system, retrieve a user input associated with the at least a slide, digitally map the user input to at least a corresponding region on the at least a slide generate at least one scanner command as a function of the transformed user input wherein the at least one scanner command is configured to command the scanning system to prioritize the transformed user input when rescanning the at least a slide and transmit the at least one scanner command to the scanning system to generate the at least a re-scanned image.

Abstract: An apparatus and method for imaging includes an imaging system formed of a movable objective stage proximal to a sample and positioned for providing an excitation beam onto and for capturing an emission from the sample. The movable objective stage includes an optical lens apparatus and a turn reflector optically coupled to the imaging optics, where at least one of the optical lens apparatus and the turn reflector are movable relative to one another for scanning the sample, and wherein the movement is achieved while maintaining a substantially fixed optical path length between the optical lens apparatus and a fixed plane in a fixed imaging optics stage.

Abstract: This application relates to the field of electrical technologies, and provides a full even harmonic digital demodulation method based on full-phase analysis for a magnetic modulator. According to the full even harmonic digital demodulation method based on full-phase analysis for a magnetic modulator, standard voltage timing data output by the magnetic modulator is obtained when a DC calibration current with a set amplitude is fed into the magnetic modulator during calibration of the magnetic modulator; an output voltage sequence from full-phase Fourier analysis is established based on the standard voltage sequence data, and the output voltage sequence is preprocessed; comprehensive analysis is performed on the preprocessed output voltage sequence from full-phase Fourier analysis to obtain all standard even harmonic signals and current conversion coefficients.

Abstract: Systems for imaging of samples, for example, using an array of micro optical elements and methods of their use. In some embodiments, an optical chip including an array of micro optical elements moves relative to an imaging window and a detector in order to scan over a sample to produce an image. A focal plane can reside within a sample or on its surface during imaging. In some embodiments, detecting optics are used to detect back-emitted light collected by an array of micro optical elements that is generated by an illumination beam impinging on a sample. In some embodiments, an imaging system has a large field of view and a large optical chip such that an entire surface of a sample can be imaged quickly. In some embodiments, a sample is accessible by a user during imaging due to the sample being exposed.

Abstract: A method is provided for operating a microscope scanner. A first imaging scan is performed of one or more area(s) of interest, AOI, on a target including a sample. This involves moving a detector array relative to the target along an image scan path and acquiring an image of the target at each of a plurality of locations along the image scan path. Focus control data is generated during the imaging scan by calculating a focus merit value at each said location along the image scan path. The focal height of the detector array is then adjusted along the image scan path based on the focus merit values. The efficacy of the first imaging scan is analysed using the focus control data and a change to one or more scanning parameters from the first imaging scan is determined, for the performance of a second imaging scan, based on this analysis.

Abstract: A method includes determining whether a variation in probe radiation intensity meets a stability criterion; directing the probe radiation through a probe, when the probe is disposed at a first position, defining a first path length L1 of the probe radiation through the fluid sample; measuring a transmitted intensity I1 of the probe radiation after passing through the fluid sample when the probe is disposed at the first position; directing the probe radiation through the probe when the probe is disposed at a second position, defining a second path length L2 of the probe radiation through the fluid sample; measuring a transmitted intensity I2 of the probe radiation after passing through the fluid sample when the probe is disposed at the second position; and determining a concentration C of a material in the fluid sample based upon L1, I1, L2, and I2, when the stability criterion is met.

Abstract: A surface inspection method includes: an irradiating step of emitting oblique illumination light onto an inspection target region of steel material using two or more oblique line light sources; an imaging step of receiving each of reflected light beams of the oblique illumination light from the respective oblique line light sources, the reflected light beams being from the inspection target region, and capturing images of the inspection target region, by one or more line sensors; and a detecting step of detecting a linear surface defect at the inspection target region using the images captured at the imaging step, wherein orthographic projections of at least two oblique illumination light beams, out of the oblique illumination light from the two or more oblique line light sources, onto a surface of the steel material are orthogonal to each other on the inspection target region.

Abstract: Embodiments of the present invention relate to an optical imaging system, and to methods, systems, and computer programs for such an optical imaging system. The methods comprise obtaining first image data of an imaging device of the imaging system, the first image data comprising a representation of a pattern. The methods comprise obtaining second image data of the pattern from the imaging device after the pattern has been displaced by a stage of the optical imaging system by a distance in a dimension defined relative to the stage. The methods comprise determining an offset between the patterns of the first and second image data in two dimensions. The methods comprise calculating a conversion parameter based on the offset and the distance. A first method comprises controlling a drive unit configured to displace the stage based on the conversion parameter.

Abstract: Provided is a calibration sample that can be used for three-dimensional structures and is unlikely to change over time. To this end, the calibration sample is a calibration sample for an autofocus target position in an optical microscope, and comprises a light-transmissive resin sample container that accommodates a first layer, which is disposed on a bottom side along the optical axis direction of the optical microscope and in which a target object having contrast with respect to a light-transmitting first resin is disposed inside the light-transmitting first resin, and a second layer which is disposed so as to cover the first layer and is composed of a light-transmitting second resin.

Abstract: An imaging device includes light sensitive locations that are separately sensitive to light received at a surface with respect to a portion of a sample associated with the surface, the light sensitive locations having a resolution of 5 microns or smaller. There is a device to associate the portion of the sample with the surface. The imaging device and a distance of the portion of the sample to the light sensitive locations are such that usable useful image of the portion of the sample can be acquired directly by operation of the imaging device. An imaging device includes light sensitive sources that are separately able to deliver light with respect to a portion of a sample associated with the surface. The light source locations having a resolution of 5 microns or smaller. There is a device to associate the portion of the sample with the surface.

Abstract: The present application provides an automatic focusing method for a fluorescence imaging device, including: S1, obtain a relationship function curve Z-Fv; S2, Gaussian fitting is performed on the relationship function curve Z-Fv, to obtain a standard Gaussian function f(x); S3, the object lens is arranged at three different travel coordinates, to obtain three groups of coordinate-clarity data (Z, Fv); S4, any two groups of the data are taken from the three groups of the coordinate-clarity data (Z, Fv) obtained in S3 and perform the Gaussian fitting with a standard deviation ?, to obtain a Gaussian function f1(x); and S5, an average value ?1 of the Gaussian function f1(x) is calculated, as a preferred travel coordinate Z? for this time of the focusing object lens. The present application solves problems of large focusing clarity error and slow focusing speed of the fluorescence imaging device in existing technologies.

Abstract: An imaging device for image generation of a specimen is disclosed. Layers of images may be captured by an optical system and then compiled to create an integrated image. Each layer may include a different focal point. A consolidated image may then be created by combining one or more integrated images.

Abstract: An optical system includes a first optical path configured to supply a first light with a first range of wavelengths; a second optical path configured to supply a second light with a second range of wavelengths shorter than the first range of wavelengths; a third optical path configured to supply a third light with a third range of wavelengths shorter than the second range of wavelengths; an optical I/O unit configured to emit the first light, the second light and the third light to a target and acquire a light from the target; a reference unit configured to split off a reference light from the third light; and a detector that includes a range of detection wavelengths shared with a CARS light and an interference light.

Abstract: A fluorescence observation apparatus includes an irradiation unit that applies a plurality of kinds of excitation light of mutually different wavelengths to a plurality of spatially or temporally different positions in a biological sample that is labeled with a composite phosphor containing two or more kinds of fluorescent molecules at a predetermined composition ratio, a detection unit that detects fluorescence generated at each of the plurality of positions by application of the irradiation unit, and a calculation unit that determines a distribution of pieces of the composite phosphor on the basis of a fluorescence signal that is obtained from a detection result of the detection unit and that shows a fluorescence intensity corresponding to a position in the biological sample of each piece of the fluorescence.

Abstract: This disclosure relates to a digital holographic microscope which is robust to an external environment. According to an aspect of the present embodiment, there is provided an optical system for a digital holographic microscope, the optical system including a beam splitter which reflects a light radiated from a light source toward an object, or passes a light reflected from and traveling from the object; an objective lens focusing the light reflected by the beam splitter on the object; a transflective mirror which is located on the surface of the objective lens and is determined to be transparent or reflective depending on a polarization direction of a light incident to the transflective mirror; and a wave plate which converts the light passing through the beam splitter into a circularly polarized light.

Abstract: Provided is an image acquisition device including: a stage on which a slide is mounted; an objective lens disposed to face a sample; a beam split unit separating light from the sample, which is incident through the objective lens into at least two, and delivering the separated lights; at least two image sensor units acquiring an image of the sample from the lights separated by the beam split unit; an image generation unit synthesizing the image acquired from the image sensor unit and generating the synthesized image; and a main control unit controlling operations of the stage or the objective lens, and the image sensor unit and the image generation unit, in which the stage is movable relatively to an optical axis of the objective lens at least in a first direction, and the at least two image sensor units acquire images at different focus positions for the sample.

Abstract: The present disclosure relates to devices, apparatus and methods of improving the accuracy of image-based assay, that uses imaging system having uncertainties or deviations (imperfection) compared with an ideal imaging system. One aspect of the present invention is to add the monitoring marks on the sample holder, with at least one of their geometric and/optical properties of the monitoring marks under predetermined and known, and taking images of the sample with the monitoring marks, and train a machine learning model using the images with the monitoring mark.

Abstract: Provision is made for a method of monitoring an immersion fluid in a microscope having a lens which images a sample located on a sample carrier. In a step a), a camera is positioned which has an image field which is oriented in such a way that it captures the sample carrier and a space between the sample carrier and the lens and adjoining the sample carrier towards the lens, which space is used to receive the immersion fluid. In a step b), the immersion fluid is applied into the space between the sample carrier and the lens. In step c), an image with the immersion fluid being in the space between the sample carrier and the lens is recorded, and in a step d), the position, the area and/or the contour of the immersion fluid on the sample carrier from the image recorded in step d) are/is determined.

Abstract: Described herein are systems and methods for assessing a biological sample. The methods include: characterizing a speckled pattern to be applied by a diffuser; positioning a biological sample relative to at least one coherent light source such that at least one coherent light source illuminates the biological sample; diffusing light produced by the at least one coherent light source; capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light; iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images; stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and identifying one or more features of the biological sample. The methods may be performed by a near-field Fourier Ptychographic system.

Abstract: A microscope includes: a motorized object stage for moving an object; an optical imaging system for forming an optical image of a plane (OE) in which the object is to be optically imaged; an optical scanning unit for moving the plane (OE) to be optically imaged by the optical imaging system relative to the optical imaging system; an image sensor for detecting the optical image of the plane (OE) formed by the optical imaging system; and a controller for controlling the motorized object stage and the optical scanning unit for simultaneously moving the object and the plane (OE) in a same direction relative to the optical imaging system while the optical image is being detected by the image sensor.

Abstract: A video processing apparatus includes: an acquisition unit configured to acquire subject eye video data; an elimination unit configured to eliminate positional offset resulting from involuntary eye movement from the subject eye video data on the basis of the subject eye video data acquired by the acquisition unit; an emphasis unit configured to perform emphasis on the subject eye video data that was subjected to the elimination performed by the elimination unit; and an output unit configured to output the subject eye video data that was subjected to the emphasis by the emphasis unit.

Abstract: A method of imaging comprises: generating a superoscillatory field from coherent electromagnetic radiation; placing an object in the superoscillatory field; detecting one or more intensity distributions of the superoscillatory field scattered by the object; and determining at least one characteristic of the object from the one or more intensity distributions.

Abstract: A method for image generation by means of a microscope includes: locating a sample to be imaged in an object space of the microscope for the image generation using specified image generation parameters; firstly detecting a movement or an intended movement of the sample; and thereupon automatically changing at least one image generation parameter during the movement of the sample depending on a movement variable.

Abstract: Provided is a particle quantitative measurement device according to which the number of particles that can be accurately recognized in a particulate sample has a wider range. An observation device 1 comprises a computer 108 and an imaging camera 107 for acquiring a sample image representing the particulate sample. As an extraction unit, the computer 108 extracts a low-brightness pixel for which I<M?k? is satisfied for brightness I from pixels of the sample image. Here, M represents brightness for a reference image, k represents a real positive number, and ? represents a standard deviation for the brightnesses of the pixels in the reference image. The computer 108 also functions as a particle recognition unit or a particle quantitative measurement unit and quantitatively measures the particulate sample on the basis of the low-brightness pixel.

Abstract: Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

Abstract: A microscope system includes an eyepiece, an objective, a tube lens, an imaging apparatus, a projection apparatus that projects a projection image onto an image plane between the tube lens and the eyepiece on which an optical image is formed, and a control apparatus. The control apparatus manages microscope information including at least a first magnification at which an image of the sample is projected onto the image plane, a second magnification at which an image of the sample is projected onto the imaging apparatus, a third magnification at which an image of the projection apparatus is projected onto the image plane, and sizes of the imaging apparatus and the projection apparatus. The control apparatus includes a processor. The processor generates projection image data representing the projection image based on at least the microscope information.

Abstract: One example system comprises a LIDAR sensor that rotates about an axis to scan an environment of the LIDAR sensor. The system also comprises one or more cameras that detect external light originating from one or more external light sources. The one or more cameras together provide a plurality of rows of sensing elements. The rows of sensing elements are aligned with the axis of rotation of the LIDAR sensor. The system also comprises a controller that operates the one or more cameras to obtain a sequence of image pixel rows. A first image pixel row in the sequence is indicative of external light detected by a first row of sensing elements during a first exposure time period. A second image pixel row in the sequence is indicative of external light detected by a second row of sensing elements during a second exposure time period.

Abstract: An indicator of a first type and an indicator of a second type are attached to a unit of a chemical component in a sample to form a first multi-indicator complex. The first multi-indicator complex includes the unit of the chemical component, the indicator of the first type, and the indicator of the second type. The indicator of the first type and the indicator of the second type have different discernible characteristics. An image of the sample, including the first multi-indicator complex corresponding to the unit of the chemical component, is captured by an image sensor. Based on a first image of the sample, a count is generated of multi-indicator complexes that include an indicator of the first type and an indicator of the second type, including the first multi-indicator complex. Based on the count, a presence or a level of the chemical component in the sample is identified.

Abstract: The present invention relates to an apparatus for inspecting the ocular fundus that comprises: —an illuminator (1) adapted to provide an illuminating light beam (IL) to illuminate a portion of said biological tissue, said illuminating beam being shaped so that at least a portion of said illuminating beam has a line-shaped section; —one or more lenses (2, 4, 7) to focus said illuminating beam (IL) on said biological tissue, during operation of said apparatus said illuminating beam illuminating a line-shaped region (5B) of said biological tissue; —a scanning assembly (3) adapted to perform optical scans of said biological tissue by cyclically moving the illuminating beam (IL) projected by said illuminator on said biological tissue, along a scanning direction (DS) substantially perpendicular to a main extension direction (AE) of the region (5B) of biological tissue illuminated by said illuminating beam; —acquisition means (6) adapted to receive reflected light (R) from said biological tissue to acquire images of

Abstract: Systems and methods for automated, non-supervised, parameter-free segmentation of single cells and other objects in images generated by fluorescence microscopy. The systems and methods relate to both improving initial image quality and to improved automatic segmentation on images. The methods will typically be performed on a digital image by a computer or processor running appropriate software stored in a memory.

Abstract: A method of providing an assembled image using a digital microscope, the digital microscope having an optical system, an image sensor having a predefined number of image pixels, and a stage, the stage being movable in relation to the optical system and the image sensor, includes receiving a user selection regarding an area of interest of the sample, the user selection indicating the position and extension of the area of interest, selecting one of a full resolution mode or and a reduced resolution mode, wherein individual images with a reduced number of image pixels are generated, moving the stage with respect to the optical system and the image sensor, and generating individual images of the area of interest in accordance with the selected one of the full resolution mode or the reduced resolution mode, and combining the individual images into the assembled image, representing the area of interest.

Abstract: An apparatus and method for imaging includes an imaging system formed of a movable objective stage proximal to a sample and positioned for providing an excitation beam onto and for capturing an emission from the sample. The movable objective stage includes an optical lens apparatus and a turn reflector optically coupled to the imaging optics, where at least one of the optical lens apparatus and the turn reflector are movable relative to one another for scanning the sample, and wherein the movement is achieved while maintaining a substantially fixed optical path length between the optical lens apparatus and a fixed plane in a fixed imaging optics stage.

Abstract: A system includes a plurality of modular subassemblies and a plate; wherein each modular subassembly comprises an enclosure and a plurality of optical components aligned to the enclosure, and each enclosure comprises a plurality of mounting structures; and wherein each modular subassembly is mechanically coupled to the plate by attachment of a mounting structure of the modular subassembly directly to a corresponding mounting structure located on the plate, such that by mechanically coupling each modular subassembly to the plate using the mounting structure of the modular subassembly and the corresponding mounting structure on the plate, adjacent modular subassemblies are aligned to each other upon such attachment, and wherein two of the modular subassemblies mechanically coupled to the plate are also attached to each other by mechanically coupling an alignment structure on one of the two modular subassemblies to a respective alignment structure on the other of the two modular subassemblies.

Abstract: A system for remotely controlling microscopic machinery and a method thereof are provided. The system includes at least one local device and a remote host for sending a control command thereto. A microslide is placed on a microscopic camera device in the local device, and the microscopic camera device captures an image of the microslide according to a capture command in the control command. The local device transmits the image to the remote host in a video format. A motorized stage moves to a next preset position according to a movement command in the control command to capture another image of the microslide. The steps of capturing and transmitting the image and the step of moving are repeated until the microslide is captured completely. The invention can solve the problem of time delay when the image captured by the remote control microscopic camera device is displayed.

Abstract: An imaging device includes an alignment platform configured to hold a cell culture plate having a plurality of wells and an imaging assembly including a plurality of imaging units, each of which is configured to image one well of the plurality of wells.

Abstract: Disclosed herein is a platform and method to quantify spatiotemporal tissue swelling during biologics injection, and to predict associated increase in the mechanical stress and interstitial fluid pressure (IFP) of tissues. Accurate measure and estimation of tissue swelling, thus, can be quantitative and predictive indicator of the IPD.

Abstract: A illumination arrangement for a microscope for illuminating a sample with a light sheet includes an illumination input configured to feed an illumination beam along an optical axis of the illumination arrangement and an illumination output which faces a sample side and is configured to output the illumination beam to the sample side. A focusing optical system is provided with a set depth of focus. At least one optical modification element is configured to geometrically modify the illumination beam. Different rays of the illumination beam intersect the optical axis within an axis intersection region at the illumination output. The axis intersection region extends over at least the depth of focus of the focusing optical system along the optical axis.

Abstract: A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.

Abstract: Embodiments described herein relate to an inspection system for illumination of optical devices. The inspection system includes a stage, a focusing lens, a light source, a reflective surface, and a camera. The inspection system is operable to provide a light to a substrate. The substrate is positioned on the inspection system such that an edge of the substrate is exposed. The inspection system focuses light to the edge such that the light propagates through the substrate. The light is coupled out of the substrate, illuminating one or more optical devices disposed on the substrate. The illumination allows the camera to capture images to be inspected. The images are inspected to detect defects of the substrate.

Abstract: A disposable staining system is provided including an insertable module configured to receive a biological sample therein, the insertable module including a well in which the biological sample is positioned; and a staining cartridge configured to receive the insertable module including the biological sample therein, the insertable module being positioned in the staining cartridge. The insertable module has first and second inclined planes on opposing sides of an upper surface of the insertable module. A sled configured to receive the insertable module has corresponding first and second inclined planes on a bottom surface thereof. The first and second inclined planes of the insertable module and the first and second inclined planes of the sled are configured to transform horizontal movement of the insertable module into the staining cartridge into a vertical movement of the sled such that the sled is pushed upward.

Abstract: The present invention concerns a method for producing a microscopic image with an extended depth of field by means of a microscope. The microscope comprises an images sensor that comprises pixels that are arranged as a matrix that is formed by lines. In a step of the method, a plurality of microscopic frames of a specimen is acquired while a focus position (z) is changed. The microscopic frames are acquired line by line. The focus position (z) is changed over a course of acquiring individuals of the microscopic frames. In a further step, parts of individuals of the acquired lines are identified. These parts sharply image the specimen. The identified parts of the lines are composed in order to form a microscopic image of the specimen with an extended depth of field. Furthermore, the present invention concerns a microscope.

Abstract: A microlens array-based ultrathin microscope is provided. The microlens array-based ultrathin microscope includes a filter unit configured to selectively transmit fluorescence manifested in a measurement sample, and an image unit configured to acquire an image from light transmitted by the filter unit. The filter unit is formed to be in contact with or spaced apart from one surface of a transparent substrate, and the image unit includes a microlens array formed on an opposite surface to the transparent substrate in which the filter unit is formed, and an image sensor configured to collect image information of the microlens array.

Abstract: A method of image evaluation when performing SIM microscopy on a sample includes: A) providing n raw images of the sample, which were each generated by illuminating the sample with an individually positioned SIM illumination pattern and imaging the sample in accordance with a point spread function, B) providing (S1) n illumination pattern functions, which each describe one of the individually positioned SIM illumination patterns, C) providing (S1) the point spread function and D) Carrying out an iteration method, which includes following iteration steps a) to e), as follows: a) providing an estimated image of the sample, b) generating simulated raw images, in each case by image processing of the estimated image using the point spread function and one of the n illumination pattern functions such that n simulated raw images are obtained, c) assigning each of the n simulated raw images to that of the n provided raw images which was generated by the illumination pattern that corresponds to the illumination patter

Abstract: Aspects of present disclosure relate to real-time image generation. An exemplary apparatus for real time image generation includes at least an optical system, a slide port configured to hold a slide, an actuator mechanism mechanically connected to a mobile element, a user interface comprising an input interface and an output interface, at least processor configured to: using the at least an optical system, capture a first image of the slide at a first position, modify the first image, using the output interface, display the first image to a user, using the input interface, receive a parameter set from the user.

Abstract: A method for acquiring annotated data with the aid of surgical microscopy systems comprises obtaining desired criteria which are intended to be satisfied by desired data to be annotated, and storing the set of desired criteria in a plurality of surgical microscopy systems. In each surgical microscopy system, images are then recorded and current criteria which correspond to the recorded images are determined. The current criteria are compared with the desired criteria. If the desired criteria sufficiently correspond to the current criteria, a confirmation is requested from a user as to whether said user would like to annotate data. If the user provides the confirmation, annotations for images are received from the user and stored together with the images.

Abstract: Described herein are systems and methods for assessing a biological sample. The methods include: characterizing a speckled pattern to be applied by a diffuser; positioning a biological sample relative to at least one coherent light source such that at least one coherent light source illuminates the biological sample; diffusing light produced by the at least one coherent light source; capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light; iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images; stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and identifying one or more features of the biological sample. The methods may be performed by a near-field Fourier Ptychographic system.